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Importance of the conserved W-X-G motif to EspK export and cell wall localization. Immunoblots of 10 μg/well each of CF, CW, CM, and cytosol proteins from espK::Tn strain complemented with pMDespKWT, pMDespKW62R, and pMDespKG64R (blot is representative of at least 2 independent experiments; L, protein ladder).
