FIG 7.

Pulldown analysis of EspB and EspK coexpressed in E. coli. (A and B) Immunoblots of EspK pulled down by His tag Dynabeads from the lysates of E. coli/His tag EspB^WT + S tag EspK^WT and E. coli/S tag EspK^WT (A) and EspB pulled down by S-protein agarose beads from the lysates of E. coli/His tag EspB^WT + S tag EspK^WT and E. coli/His tag EspB^WT (B). (C and D) Immunoblots of EspB and EspK pulled down by His tag Dynabeads (C) and S-protein agarose beads (D) from the lysates of E. coli/His tag EspB^WT + S tag EspK^WT, E. coli/His tag EspB^WT + S tag EspK^W62R, and E. coli/His tag EspB^WT + S tag EspK^G64R (each set of blots is representative of at least 2 independent experiments; I, input 5 μg of lysates; E, all proteins eluted from beads in 50 μL of SDS-PAGE sample buffer).