FIG 8.

EspB native conformation analysis. (A and B) Native PAGE and EspB immunoblots of CL proteins (5 μg/well) from wild-type M. tuberculosis Erdman (WT) and espK::Tn strain cultured for 3 days in Sauton’s medium without Tween 80 (A) and CF protein (10 μg/well) of espK::Tn complemented with pMDespK^WT and CL proteins (5 μg/well) of espK::Tn complemented with pMDespK^WT, pMDespK^W62R, pMDespK^G64R, and pMD31 cultured for 4 days in Sauton’s medium without detergent (B) (each set of blots are representative of at least 2 independent experiments; asterisks indicate higher-order oligomers of EspB).