. 2022 Mar 14;204(4):e00611-21. doi: 10.1128/jb.00611-21

TABLE 3.

Interaction of Pol IV with SSB^a

Interaction^b	Expt	K_D (nM)	Avg K_D, nM (range)
Interaction^b	Expt	K_D (nM)	K_D ₁	K_D ₂
Pol IV-SSB^His	1	K_D₁ = 0.33	0.47 (0.27)	11.6 (1.40)
		K_D₂ = 10.9
	2	K_D₁ = 0.60
		K_D₂ = 12.3

Pol IV-T120P–SSB^His	1	K_D₁ = 42.5	37.7 (9.6)	70.3 (7.4)
		K_D₂ = 74.0
	2	K_D₁ = 32.9
		K_D₂ = 66.6

Pol IV^CD-SSB^His	1	K_D₁ = 28.1	28.5 (0.70)	340 (52.0)
		K_D₂ = 314
	2	K_D₁ = 28.8
		K_D₂ = 366

Pol IV^CD-T120P–SSB^His	1	K_D₁ = 2,190	1,845 (690)	3,095 (490)
		K_D₂ = 3,340
	2	K_D₁ = 1,500
		K_D₂ = 2,850

Pol IV^LF-SSB^His	1, 2	ND^c	ND	ND

See Table S3 in the supplemental material for the k_a (on-rate), k_d (off-rate), χ² (likelihood of no relationship), and R² (goodness of fit) values for these interactions.

The indicated His-tagged protein (ligand) was captured on a HIS1K biosensor, and interactions were measured using BLI.

ND, no interaction was detected.