FIGURE 3.

L-Theanine and Mg-L-theanine compounds reverse caffeine’s effect on inhibitory and glutamate receptors. Western blot results of GABAergic receptors GABA_A-R (A), GABA_B-R1 (B), GABA_B-R2 (C), serotonergic receptor 5-HT_1A (D), glutamatergic receptors GluA1 (E), GluN1 (F), GluN2A (G), and nitric oxide synthases, eNOS (H), and iNOS (I). All groups normalized according to housekeeping protein β-actin (three β-actin blots were performed for GABAergic and serotonergic receptors; glutamatergic receptors; and NOS), and one-way ANOVA with Tukey’s HSD was performed for multiple comparisons. Values are represented as mean ± SD. *p < 0.05: compared to control, ^#p < 0.05: compared to caffeine control, ^$p < 0.05: compared to L-theanine, ^¥p < 0.05: compared to Mg-T1.