FIG 1.

Cidality of compound 22. (A) Dose-response MICs for isoniazid, linezolid, and moxifloxacin and 22 against H37Rv over 7 days in Middlebrook 7H9 supplemented with OADC and glycerol. (B) MBC determinations for the same compounds. (C and D) Time- and concentration-dependent kill of replicating M. tuberculosis H37Rv by 22 (C) and moxifloxacin (D). The effect on viability was assessed by plating serial dilutions on standard Middlebrook 7H10 agar (supplemented with glycerol, OADC, and charcoal) at the indicated times followed by scoring CFU. Here, the MIC represents the liquid broth MIC. The results represent the means with standard deviations, and the limit of detection (LOD) is 10 bacilli.