FIG 4.

Disruption of the Rcs system restored motility. First, 5 μL of cells of each indicated strain grown the mid-log phase was spotted onto the swimming plate (tryptone, 10 g/L; yeast, 5 g/L; NaCl, 10 g/L; agar, 0.2%) and was dried at room temperature for 5 min followed by incubation at 37°C for 7 h. The assay was performed in 5 replicates for each strain. The representative image is presented on the left. The swimming diameter is measured and shown as the mean ± standard deviation on the right. ***, P < 0.001.