TABLE 3.
Bacterial strains and plasmids used in this study
| Name | Description | Reference or source |
|---|---|---|
| Strains | ||
| ExPEC PCN033 | A virulent clinical strain isolated from diseased pig, wild type | 72 |
| E. coli χ7213 | A diaminopimelic acid autotrophic E. coli strain used for transconjugation | 73 |
| Δtat | As ExPEC PCN033, tatABC deleted | 25 |
| CΔtat | Δtat strain expressing tatABC from a plasmid | |
| ΔmodD | As ExPEC PCN033, modD deleted | |
| ΔamiA | As ExPEC PCN033, amiA deleted | |
| ΔamiC | As ExPEC PCN033, amiC deleted | |
| ΔefeOB | As ExPEC PCN033, efeOB deleted | |
| ΔsufI | As ExPEC PCN033, sufI deleted | |
| ΔmepK | As ExPEC PCN033, mepK (previously known as ycbK) deleted | |
| ΔfhuD | As ExPEC PCN033, fhuD deleted | |
| ΔmoaA | As ExPEC PCN033, moaA deleted | |
| ΔnrfC | As ExPEC PCN033, nrfC deleted | |
| ΔhybOA | As ExPEC PCN033, hybOA deleted | |
| ΔyagT | As ExPEC PCN033, yagT deleted | |
| ΔyahJ | As ExPEC PCN033, yahJ deleted | |
| ΔhyaA | As ExPEC PCN033, hyaA deleted | |
| ΔfdoG | As ExPEC PCN033, fdoG deleted | |
| ΔwacM | As ExPEC PCN033, wcaM deleted | |
| ΔcueO | As ExPEC PCN033, cueO deleted | |
| ΔnapG | As ExPEC PCN033, napG deleted | |
| ΔydhT | As ExPEC PCN033, ydhT deleted | |
| ΔydhX | As ExPEC PCN033, ydhX deleted | |
| ΔfdnG | As ExPEC PCN033, fdnG deleted | |
| ΔamiAΔamiC | As ExPEC PCN033, amiA and amiC deleted | |
| ΔrcsDB | As ExPEC PCN033, rcsDB deleted | This study |
| ΔtatΔrcsDB | As Δtat, rcsDB deleted | This study |
| ΔmdoDΔrcsDB | As ΔmdoD, rcsDB deleted | This study |
| ΔamiAΔamiCΔrcsDB | As ΔamiAΔamiC, rcsDB deleted | |
| ΔtatΔcpxAR | As Δtat, cpxAR deleted | This study |
| Plasmids | ||
| pRE112 | The plasmid used in mutant construction | Lab stock |
| pHSG396-Apra | The plasmid used for in trans protein expression in ExPEC | This study |
| pHSG396-Apra-MdoD | As pHSG396-Apra, a DNA fragment containing a tat promoter followed by the coding sequence of mdoD followed by insertion between XbaI and EcoRI | This study |
| pHSG396-Apra-MdoDRR-KK | As pHSG396-Apra-MdoD, expressing the variant MdoD, where the R3 and R4 residues were both replaced with a lysine | This study |
| pHSG396-Apra-RcsDB | As pHSG396-Apra, a DNA fragment containing a tat promoter followed by the coding sequence of rcsDB inserted between XbaI and EcoRI | This study |
| pHSG396-Apra-AmiA/AmiC | As pHSG396-Apra, a DNA fragment containing the coding sequence of rcsDB followed by a tat promoter inserted between kpnI and SacI | This study |
| pRE112-rcsDB | As pRE112, a DNA fragment containing the upstream 1,000 bp and downstream 1,000 bp of rcsDB inserted between kpnI and SacI | This study |
| pRE112-cpxAR | As pRE112, a DNA fragment containing the upstream 1,000 bp and downstream 1,000 bp of cpxAR inserted between kpnI and SacI | This study |