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. 2022 Apr 15;15:691–701. doi: 10.2147/CCID.S362785

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© 2022 Chen et al.

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Primary fibroblasts were treated with the vehicle, AF and Res for 1 month. (A) The EdU incorporation assays were performed to determine the proliferation. Representative images (left) and the statistical analysis (right) for the rate of the Edu positive cells in different groups, bar = 50 µm (* = P < 0.05). (B) CCK-8 assay was used to detect the viability of fibroblast in different groups. (* = P < 0.05). (C) Representative images of SA-β-gal staining in different groups, bar = 50 µm. (D) SA-β-gal activity was measured by using activity assay kit in different groups (* = P < 0.05). Results are shown as means ± SEM.