Figure 1.

Diagram of the complete experimental schedule. After bilateral olfactory bulbectomy, all animals had a recovery period of 14 days, and then we determine their hyperactivity using the open field test. (A) In the first set of animals, we analyzed the mRNA levels of the receptors involved in our studies. (B). In a second set of animals, we evaluated the effects of different pharmacological treatments in the forced swimming test (FST) or in the sucrose preference test (SPT). One week after the behavioral tests, we collected the blood and brain of the animals to perform the experiments of autoradiography and in situ hybridization and to determine the blood levels of corticosterone.