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. 2022 Apr 19;5(8):e202101145. doi: 10.26508/lsa.202101145

Figure 7. Survival motor neuron (SMN)–deficient motor neurons exhibit reduced DDX21 levels in a cell autonomous manner.

Figure 7.

(A) p75 enriched motor neurons derived from SMNΔ7 and wild type E13 embryos were labelled with DDX21 antibody, at DIV7. Scale bars represent 5 μm. (B, C, D) Nuclear, (C) nucleoplasmic and (D) foci fluorescence intensity of DDX21 staining normalised to control. Bar graphs of mean ± s.d. *P < 0.05; paired two-tailed t test (P = 0.0417). (E) iPSC-derived motor neurons isolated from healthy individuals (csi4i and miff) and spinal muscular atrophy (SMA) type I patients (SMA86 and SMA32) were labelled with DDX21 antibody. Scale bars represent 5 μm. (F) Nuclear fluorescence intensity of DDX21 staining normalised to the average of control samples. (G) Nuclear fluorescence intensity where control samples (csi4i and miff) and SMA samples (SMA68 and SMA32) have been pulled together. (H) Nucleoplasmic fluorescence intensity of DDX21 staining normalised to the average of control samples. (I) Nucleoplasmic fluorescence intensity where control samples (csi4i and miff) and SMA samples (SMA68 and SMA32) have been pulled together. (J) Foci fluorescence intensity of DDX21 staining normalised to the average of control samples. (K) Foci fluorescence intensity where control samples (csi4i, miff) and SMA samples (SMA68, SMA32) have been pulled together. Bar graphs of mean ± s.d. *P < 0.05; paired two-tailed t test (P = 0.0344).