Skip to main content
. 2022 Apr 19;5(8):e202101145. doi: 10.26508/lsa.202101145

Figure 8. DDX21 levels in spinal muscular atrophy (SMA) embryonic cortical neurons and fibroblasts derived from SMA patients.

Figure 8.

(A) SMA and control embryonic cortical neurons were labelled with DDX21 antibody, at DIV7. Scale bars represent 5 μm. (B, C, D) Nuclear (B), nucleoplasmic (C), and foci (D) fluorescence intensity of DDX21 staining is presented normalised to control. Bar graphs of mean ± s.d (N = 3). ns, not significant (P > 0.05). (E) Fibroblasts derived from SMA type I patient (SMA) and a healthy individual (control) were doubled-stained with DDX21 and cyclin A1. Scale bars represent 10 μm. Cyclin A1–positive cells were excluded from the analysis. (F, G, H) Nuclear (F), nucleoplasmic (G), and foci (H) fluorescence intensity of DDX21 staining is presented normalised to control. Bar graphs of mean ± s.d (N = 3). ns, not significant (P > 0.05).