Figure 2. The tricalbins regulate phosphatidylserine homeostasis at the plasma membrane.

(A) Schematic representation of PS, PE, and PC production and the enzymes involved. (B) PS FLARE (GFP-Lact-C2) localisation in wild type, tcb1/2/3Δ, scs2/22Δ ist2Δ, and Δtether cells. Scale bars, 4 μm. (C) Quantitation of relative GFP-Lact-C2 intensity at the plasma membrane of the mother cell. Data represent mean ± SD. Total number of cells analysed in three independent experiments: wild type n = 196, tcb1/2/3Δ n = 194, scs2/22Δ ist2Δ n = 187, Δtether n = 184. ****P > 0.0001. (D) Lipidomic analysis of total PS, PE and PC species in wild type, tcb1/2/3Δ, scs2/22Δ ist2Δ, and Δtether cells. Data represent mean ± SD (n = 3). *P > 0.1; ns, not significant. (E) The fatty acid degree of unsaturation for PS, PE, and PC species in wild-type, tcb1/2/3Δ, scs2/22Δ ist2Δ, and Δtether cells. Data represent mean ± SD (n = 3). ***P > 0.001, **P > 0.01, *P > 0.1; ns, not significant. Also see Fig S2.

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