Table 1.
Steady-state kinetic parameters of Copu9 and its variants calculated from the EnzChek™ pyrophosphate assay
| Copu9 | kcat [s−1] | Km [µM] | kcat/Km (× 103) [s−1 M−1] |
|---|---|---|---|
| WT | (3.24 ± 0.03) × 10–2 | 4.59 ± 0.17 | 7.067 |
| T73C | (5.02 ± 0.26) × 10–2 | 6.28 ± 1.37 | 7.995 |
| C76V | (2.91 ± 0.07) × 10–2 | 3.14 ± 0.45 | 9.252 |
| T192N | (3.96 ± 0.08) × 10–2 | 8.28 ± 0.60 | 4.782 |
| G194A | (3.60 ± 0.05) × 10–2 | 7.00 ± 0.37 | 5.135 |
| C195V | (3.98 ± 0.07) × 10–2 | 8.48 ± 0.51 | 4.692 |
| P197C | (3.81 ± 0.10) × 10–2 | 6.82 ± 0.77 | 5.584 |
| N228A | (2.62 ± 0.07) × 10–2 | 4.42 ± 0.59 | 5.940 |
| S314N | (1.92 ± 0.05) × 10–2 | 5.10 ± 0.54 | 3.755 |