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. 2022 Mar 24;11:e74877. doi: 10.7554/eLife.74877

Figure 2. Translational rescue experiments in the presence of montelukast and saquinavir.

Figure 2.

(A) Luciferase-based reporter assay shows translational rescue of luciferase in the presence of montelukast. (B) Luciferase-based reporter assay shows that saquinavir could not rescue the luciferase expression. Error bars represent standard deviation of three replicates in (A) and (B). (C and D) The real-time PCR to quantitate the fold change of FLuc gene in comparison to GAPDH in the presence of different concentration of the drug molecules. (A) montelukast (B) saquinavir. The panel below provides the details of experimental conditions. Error bars represent standard deviation of three replicates in (A) and (B). The significance of the data was monitored by applying the unpaired t-test through assuming Gaussian distribution parametric test by defining the statistical significance. **p < 0.01; ***p < 0.001; ****p < 0.0001. The error bars represent the standard deviation.