Table 1. Anaphase B microtubule dynamics.
Mean and standard deviation (S.D.) for several magnitudes of microtubule dynamics measured from kymographs of cells expressing Alp7-3xGFP, Sid4-GFP, and Cut11-mCherry. The microtubule growth speed is categorised according to whether rescue occurred before the dumbbell transition, and inside or outside the nuclear membrane bridge (see cartoons in Figure 3). Data from cells grown in YE5S medium and imaged at 27 °C in the absence of DMSO (wild-type in Figure 5F and Figure 4—figure supplement 2F).
| Mean ± S.D. | |
|---|---|
| Growth speed before | 1.54 ± 0.31 μm/min |
| Growth speed outside | 1.37 ± 0.31 μm/min |
| Growth speed inside | 0.66 ± 0.44 μm/min |
| Growth event duration | 49 ± 19 s |
| Position of plus-end with respect to spindle center at rescue | 1.02 ± 0.48 μm |
| Shrinkage speed | 4.12 ± 1.56 μm/min |