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a) Superimposed distances (in Angstroms) between donor nitrogen (NE) of R195 with acceptor oxygen (O) of Y221 in (MMP1 Zn1 Zn2) (red), (MMP1 Zn1) (blue), (MMP1 Zn2) (yellow), and (MMP1 no Zn) (green). The R195 – Y221 hydrogen bond is lost when the Zn(II) ion is removed from the structural site. b) Distance between donor nitrogen (NH2) of R195 with acceptor oxygen (O) of S220 in (MMP1 Zn1 Zn2) (red), (MMP1 Zn1) (blue), (MMP1 Zn2) (yellow), and (MMP1 no Zn) (green).
