Fig. 5.

Identification of stemness subtypes of LUAD single cells in LUAD-scRNA. A. Hierarchical clustering identifying three stemness subtypes of LUAD single cells. Comparisons of the enrichment scores of three biological processes (B), expression levels of DNA damage response genes (C), and inferred CNVs by inferCNV [27] (D) among the stemness subtypes of LUAD single cells. E. Clustering analyses of LUAD single cells by SC3 [25] identifying 14, 15, and 18 cell clusters in St-H, St-M, and St-L, respectively. F. Pseudotime analysis showing the trajectory paths of the stemness subtypes of LUAD single cells. G. Gene set enrichment analysis by Monocle2 [24] identifying the gene ontology (GO) biological process (BP) terms enriched in the stemness subtypes of LUAD single cells. The one-tailed Mann–Whitney U test (B) and one-way ANOVA test (C) P-values are shown. *** P < 0.001.