Figure 1.

Pedigree of the family. Twenty-two individuals (17 related family members and 5 spouses) were recruited by cascade screening through the index patient (blue arrow). Black symbols denote a high-Lp(a) (lipoprotein [a]) phenotype (>150 nmol/L) and white symbols normal Lp(a) phenotypes (≤90 nmol/L). Below each symbol, the first line displays individual ID numbers, the second line plasma Lp(a) concentrations (in nmol/L), and the third line the size of their apo(a) isoforms (number of KIV [kringle IV] domains). Individuals with established premature coronary artery disease are framed. Not avialable, as isoform expression is too low for detection. For each individual (n=22), plasma lipids and lipoproteins concentrations, including Lp(a) were assessed in plasma samples at least three times independently. Western blots used to determine apo(a) isoform sizes were performed twice on each plasma sample in 2 independent experiments. A representative Western Blot is displayed (inset).