Fig. 3.

(A) Black-stained reticular fibers surrounded individual tumor cells or tumor cell nests (silver impregnation. Scale bar: 100 µm). (B) In the normal spleen, reticular fibers were arranged in a stratiform pattern in the periarterial lymphoid sheath (PALS), were sparse in the lymphoid follicle (LF), and arranged in a mech structure in the marginal zone (MZ) and red pulp (RP) (silver impregnation. Scale bar: 100 µm). (C) The majority of the tumor cells stained positive for fascin (scale bar: 100 µm). (D) The tumor cells stained positive for desmin (scale bar: 100 µm). (E) Many non-tumor stellate-shaped cells were positive for Iba-1 (scale bar: 100 µm). (F) Double staining for fascin (red) and desmin (green). Numerous double positive tumor cells (yellow) were observed.