Fig. 3.

Inhibition of TLR4/MD-2 can partially reduce the activation of KCs.

Upon treatment with TLR4 and MD-2 antibodies, the activity of KCs and the secretion of pro-inflammatory factors were partially inhibited in the liver. (A) Expression of the KC marker, CD68, was observed through immunofluorescence, revealing that the antibodies of TLR4/MD-2 can partially inhibit the expression level of CD68. (B) Western blotting and (C) RT-qPCR were used to estimate the levels of CD68 and pro-inflammatory factors TNF-α, IL-1β, and MIP-1α. The results showed that the inhibition of TLR4/MD-2 can partially inhibit the expression of these factors. The scale bar is 100 μm. ***p<0.001 vs. the control group. ^#p<0.05, ^###p<0.001, vs. the EtOH group.