Reagent	Final concentration	Amount
NaCl (5 M)	150 mM	1.5 mL
HEPES-KOH (1 M, pH 8.0)	50 mM	2.5 mL
MgCl2 (1 M)	5 mM	0.25 mL
DTT (1 M)	2 mM	0.1 mL
Deionized water	n/a	45.65 mL