| Reagent | Final concentration | Amount |
| 10× TBE | 1× | 50 mL |
| 40% Acrylamide/bis-acrylamide (19:1) | 15% | 187.5 mL |
| Urea | 8 M | 240.24 g |
| Deionized water | n/a | Add to 500 mL in total |
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