DMEM Maintenance Media (DMEM GM)
| Reagent | Final concentration | Final concentration |
|---|---|---|
| DMEM with L-glutamine and sodium pyruvate | 96% | 480 mL |
| Heat inactivated FBS | 2% | 10 mL |
| Hepes | 1% | 5 mL |
| Penicillin/Streptomycin | 1% | 5 mL |
| Total | N/A | 500 mL |
Store at 4°C for up to 1 month.