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2× DMEM media
| Reagent | Amount |
|---|---|
| DMEM, powder, high glucose | 1 pack |
| Distilled water | 470 mL |
| Heat inactivated FBS | 20 mL |
| Hepes | 10 mL |
| Penicillin/Streptomycin | 10 mL |
| Total | 500 mL |
Sterilize by passing media through the 0.2 μm filter. Store at 4°C for up to 1 month.
