REAGENT or RESOURCE	SOURCE	IDENTIFIER
Experimental models: Cell lines
Vero cells	ATCC	Cat# CCL-81
MRC-5 cells	ATCC	Cat# CCL-171
HEK293 cells	ATCC	Cat# CRL-1573
A549 cells	ATCC	Cat# CRM-CCL-185
Bacterial and virus strains
Zika MR766, GENBANK: M143022.1	ATCC	Cat #VR-1838
Zika H/PF/2013, GENBANK: KJ776791.2	European Virus Archive	N/A
Zika DN-2, Virus made from PF13/251013-18 (GENBANK: KX369547) as viral backbone	Derived from infectious clone (Kwek et al., 2018)	N/A
Zika DN-1, Virus made from PF13/251013-18 (GENBANK: KX369547) as viral backbone	Derived from infectious clone (Kwek et al., 2018)	N/A
Zika M-F37L, Virus made from PF13/251013-18 (GENBANK: KX369547) as viral backbone	Derived from infectious clone (Yau et al., 2021)	N/A
Dengue virus	Isolated from patient (Low et al., 2006)	N/A
Chemicals, peptides, and recombinant proteins
DMEM with L-glutamine and Sodium Pyruvate	Gibco, Thermo Fisher Scientific	Cat# 11995
Fetal Bovine Serum (FBS)	HyClone	Cat# SH30396.03
1 M Hepes	Lonza	Cat# 17-737E
Penicillin/Streptomycin (Pen/Strep)	HyClone	Cat# SV30010
MEM with L-glutamine	Gibco, Thermo Fisher Scientific	Cat# 11095
L-glutamine (100×)	Gibco, Thermo Fisher Scientific	Cat# 25030081
DMEM, powder, high glucose	Gibco, Thermo Fisher Scientific	Cat# 12100046
Aquacide II (Carboxymethyl cellulose)	Merck Millipore	Cat# 17851
Trypsin-EDTA (0.25%), Phenol Red	Gibco, Thermo Fisher Scientific	Cat# 25200072
Trypan Blue	Sigma-Aldrich	Cat# 15250061
Crystal Violet Solution	Sigma-Aldrich	Cat# V5265
10× PBS	Sigma-Aldrich	Cat# BUF-2040-10X4L
0.22 μM filter	Nalgene	Cat# 595-4520
Oligomycin A	Sigma-Aldrich	Cat# 209-437-3
Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone (FCCP)	Sigma-Aldrich	Cat# 206-730-8
Rotenone	Sigma-Aldrich	Cat# 83-79-4
Antimycin A from Streptomyces sp.	Sigma-Aldrich	Cat# A8674
Glucose	Sigma-Aldrich	Cat# G7021
Sodium pyruvate	Sigma-Aldrich	Cat# 5280
Glutamine	Sigma-Aldrich	Cat# G8540
2-deoxy-D-glucose	Sigma-Aldrich	Cat# D8375
Critical commercial assays
Seahorse XF24 V28 PS Cell Culture Microplates	Agilent	Cat# 100882-004
Seahorse XF24 Islet Capture Microplates	Agilent	Cat# 101122-100
Seahorse XF RPMI Medium, pH 7.4, 500 mL	Agilent	Cat# 103576-100
Seahorse XF Calibrant Solution	Agilent	100 mL (Cat# 103059-000) or 500 mL (Cat# 100840-000)
Seahorse XFe24 Analyzer	Agilent	N/A
Software and algorithms
Seahorse Wave Desktop Software	Agilent	N/A