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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Biol Psychiatry. 2022 Jan 11;91(9):832–840. doi: 10.1016/j.biopsych.2021.12.018

Figure 4. Optoinhibition of the BLA reduces freezing to both recent and remote CSs in a within-subjects fear conditioning procedure.

Figure 4.

A, Schematic representation of the experimental design. 8 kHz and 2 kHz tones were used as remote (CSremote) and recent (CSrecent) cues, respectively. Continuous laser illumination started 10 s before the first tone onset and persisted to the end of testing session. B, Jaws silencing decreased spontaneous firing rate of BLA neurons. C, Micrograph showing viral expression and fiber placement in the BLA. D, Recent fear conditioning. E, Optoinhibition of the BLA dramatically reduced CSrecent-induced freezing. Each trial consisted of a 10 s CS and a 30 s post-CS period. F, Average freezing during recent fear retrieval. G, Remote fear conditioning. H, Optoinhibition of the BLA also decreased CSremote-induced freezing. I, Average freezing during remote fear retrieval. Data are shown as means ± SEMs. *p < 0.05, n = 11-12 per group. BL, baseline; BLA, basolateral amygdala; CS, conditioned stimulus.