Figure 2.

LmHsp100_N features the methionine-aromatic amino acid motif that protects the N-domain from oxidative damage. (A) Enlarged view of the hydrophobic cleft showing the location of the six methionines (green) as well as of Trp8 and Phe111 (purple), with the tryptophan side chain being solvent exposed. (B) Phe111 is surrounded by methionine residues that protect this critical residue from oxidative damage. Four out of five methionines are shown for clarity. A section of the composite 2Fo-Fc electron density map is shown as purple mesh and contoured at the 1.0 σ level.