Figure 7.

Quantification of glial cells and processes

Adult mice were injected SR with 3.0 × 10⁷ vg/eye of AAV-PHP.eB-CMV-EGFP (SR AAV-PHP.eB) or 3.0 × 10⁷ vg/eye of AAV2/8-CMV-EGFP (SR AAV2/8), or via the TV with 5.0 × 10¹⁰ vg of AAV-PHP.eB-CMV-EGFP (TV AAV-PHP.eB), or IVT with 7.5 × 10⁸ vg/eye of AAV-PHP.eB-CMV-EGFP (IVT AAV-PHP.eB) or 7.5 × 10⁸ vg/eye of AAV2/2-CMV-EGFP (AAV2/2); uninjected eyes were used as controls. Eyes were fixed in 4% PFA at 1 month post-injection and cryosectioned. Sections were labeled with IBA1 and GFAP immunohistochemistries. IBA1⁺ cell bodies (A) and GFAP⁺ processes in the IPL were quantified using CellSens. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ANOVA.