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. Author manuscript; available in PMC: 2022 Oct 15.
Published in final edited form as: Cancer Res. 2022 Apr 15;82(8):1518–1533. doi: 10.1158/0008-5472.CAN-21-1807

Figure 3. Epigenetic downregulation of APC promotes tumor growth but does not correlate with Wnt/β-catenin pathway activation.

Figure 3.

A, Expression correlation analysis of APC with promoter methylation in TCGA (n=436, cases with genomic APC alterations excluded). B, Kaplan Meier survival analysis of cases with different APC expression levels in MSKCC (Memorial Sloan Kettering Cancer Center) and TCGA datasets. Left panel: MSKCC (Unaltered group represents APC expression z>0.7, n=12; Altered group represents APC expression z<−1.7, n=11). Right panel: TCGA (Unaltered group represents APC expression z>0.7, n=62; Altered group represents APC expression z<−0.7, n=86). Statistics: Log-rank Test performed. C, Left panel: Growth curves of xenografts derived from RV1 cells with either doxycycline-inducible shAPC (RV1_shAPC_DOX, red curves) or shNTC (non-target control, RV1_shNTC_DOX, black curves). Middle and right panels: qRT-PCR for APC (middle panel) or AXIN2 (right panel) in RV1_shAPC_DOX (red dots) and RV1_shNTC_DOX (black dots) harvested at indicated endpoints. D, Expression correlation of APC with WNT activity scores in TCGA (n=548), FHCRC (n=149), SU2C (n=89) and WCPCDT (n=99) datasets. E, Expression correlation of APC with cell cycle genes PLK1, BUB1, CDK6 and CDC25C in FHCRC (n=149) dataset. A, D and E statistics: Pearson correlation and linear regression. C statistics: Mann-Whitney test. P<0.05 deemed significant.