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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Nat Struct Mol Biol. 2022 Mar 24;29(4):292–305. doi: 10.1038/s41594-022-00745-3

Extended Data Fig. 7. Tau PFFs fail to induce tau pathology in Tau−/− mice.

Extended Data Fig. 7

Pre-formed fibrils (PFFs) of Tau or Tau K353R were injected into the LC of 3-month-old Tau −/− mice. The mice were assessed for DOPEGAL modification and phosphorylation of Tau 3 months later. A, B. Representative images of immunofluorescent staining for Tau K353-DOPEGAL (red); DBH (green); DAPI (blue) (A) and Tau K353-DOPEGAL (green); AT8 (red); DAPI (blue) (B). Scale bars are 100 μm (left) and 20 μm (right). C. Quantification of DBH+ cells in the LC sections of PBS, Tau, or Tau K353R PFFs-injected Tau−/− mice. Data are shown as mean ± SEM. n = 8 per group. One-way ANOVA. D. Quantification of Tau K353-DOPEGAL+ and AT8+ cells in the LC sections of PFFs-injected Tau −/− mice. Data are shown as mean ± SEM. n = 6 per group. Two-way ANOVA with Sidak’s multiple comparison.