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. Author manuscript; available in PMC: 2023 May 1.

Published in final edited form as: Food Chem Toxicol. 2022 Mar 19;163:112923. doi: 10.1016/j.fct.2022.112923

Figure 2. — (A) In the control, neutrophils are localized around the vascular niche of the caudal hematopoietic tissue (CHT) at resting state during normal development in transgenic Tg(lysC:dsRed) zebrafish. Activation of neutrophils, i.e., by the Mint e-liquid promotes migration from the vascular niche of the CHT, a well-known hallmark for a pro-inflammatory response. (B) Pattern analysis, i.e., the summation of all Nearest Neighbor Distances, is an indicator of dispersed neutrophils. Boxes in (A) indicate the posterior trunk/tail region of zebrafish embryos. (C) Quantification of the neutrophil migration assay data from panel (A). n = 10–12 zebrafish embryos per treatment group.

Figure 2. — (A) In the control, neutrophils are localized around the vascular niche of the caudal hematopoietic tissue (CHT) at resting state during normal development in transgenic Tg(lysC:dsRed) zebrafish. Activation of neutrophils, i.e., by the Mint e-liquid promotes migration from the vascular niche of the CHT, a well-known hallmark for a pro-inflammatory response. (B) Pattern analysis, i.e., the summation of all Nearest Neighbor Distances, is an indicator of dispersed neutrophils. Boxes in (A) indicate the posterior trunk/tail region of zebrafish embryos. (C) Quantification of the neutrophil migration assay data from panel (A). n = 10–12 zebrafish embryos per treatment group.