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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Food Chem Toxicol. 2022 Mar 19;163:112923. doi: 10.1016/j.fct.2022.112923

Figure 3. E-liquid exposures result in pro-inflammatory responses in zebrafish embryos.

Figure 3.

Figure 3.

(A) Pro-inflammatory responses with increases in neutrophil migration in transgenic Tg(lyscC:dsRed) zebrafish embryos are observed after exposures (3 dpf to 5 dpf) to Virginia Tobacco (VT), Menthol and Mint e-liquids or the known pro-inflammatory chemicals DSS or TNBS. Fluorescent neutrophils were imaged and analyzed using ImageXpress Pico. (B) The cell distance indicates the total nearest neighbor distances from neutrophils in the posterior trunk/tail regions of the transgenic Tg(lysC:dsRed) zebrafish at 5 dpf. n = 10–12 zebrafish embryos per treatment group. # p<0.001; * p<0.0001.