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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Nat Struct Mol Biol. 2022 Mar 24;29(4):348–356. doi: 10.1038/s41594-022-00748-0

Extended Data Fig. 7. Effects of catalytic site mutations.

Extended Data Fig. 7

a) Immunofluorescence microscopy of transfected HeLa cells shows the N406A and D408A mutants to be localized to the Golgi similar to WT PTase. b) Immunoblot of WT PTase, N406A and D408A expressed in GNPTAB−/− HeLa cells and probed with a monoclonal antibody against the V5 tag. 25μg of each cell lysate was loaded. c) α-methylmannoside activity of WT PTase, N406A and D408A expressed in GNPTAB−/− HeLa cells. The data shown are the mean ± SD for 2 independent experiments. Uncropped images for b and data for graph in c are available as source data.