Table 1.
Active state (EMBD-24784) (PDB 7S05) |
Autoinhibited state (EMBD-24785) (PDB 7S06) |
|
---|---|---|
Data collection and processing | ||
Magnification | 130,000 | |
Voltage (kV) | 300 | |
Electron exposure (e−/Å2) | 66 | |
Defocus rang (μm) | 1.0–2.3 | |
Pixel size (Å) | 0.828 | |
Symmetry imposed | C2 | |
Initial particle images (No.) | 5,186,047 | |
Final particle images (No.) | 365,927 | 66,173 |
Map resolution (Å) | 3.1 | 3.3 |
FSC threshold | 0.143 | 0.143 |
Map resolution range (Å) | 2.0 – 5.0 | 2.0 – 5.0 |
Refinement | ||
Initial model used (PDB code) | -- | -- |
Model resolution (Å) | 3.2 | 3.5 |
FSC threshold | 0.5 | 0.5 |
Model resolution range (Å) | 2–5 | 2–5 |
Map sharpening B factor (Å2) | −150.5 | −141 |
Model composition | ||
Non-hydrogen atoms | 7504 | 7850 |
Protein residues | 874 | 918 |
Ligands | 16 | 14 |
B factors (Å2) | ||
Protein | 58.4 | 90.2 |
Ligand | 81.7 | 123.2 |
R.m.s. deviations | ||
Bond lengths (Å) | 0.003 | 0.003 |
Bond angles (°) | 0.653 | 0.620 |
Validation | ||
Molprobity score | 1.79 | 1.82 |
Clashscore | 7.23 | 7.56 |
Poor rotamers (%) | 0 | 0 |
Ramachandran plot | ||
Favored (%) | 94.17 | 93.99 |
Allowed (%) | 5.83 | 6.01 |
Disallowed (%) | 0 | 0 |