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. 2022 Apr 19;13(4):376. doi: 10.1038/s41419-022-04832-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Schematic illustrating the putative E2F1-binding sites in miR-532 promoter. B, C qRT-PCR analysis of miR-532 or pre-miR-532 levels in MKN-45 cells after changing E2F1 expression. D qRT-PCR analysis of CLCN5 mRNA levels in MKN-45 cells after changing E2F1 expression.(E, F Direct binding of E2F1 to miR-532 promoter as indicated by ChIP assays. Binding was confirmed by semi-quantitative PCR, followed by gel electrophoresis. G Luciferase reporter assay confirmed the suppression of miR-532 promoter by E2F1. *P < 0.05; **P < 0.01; ***P < 0.001.