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. 2022 Apr 19;13:2055. doi: 10.1038/s41467-022-29648-z

Fig. 1. Phenotypes and identification of the wenmin1 allele.

Fig. 1

a Pollen fertility of ZJ1, Tian1S and ZJ1-wenmin1 at the indicated day average temperature (DAT). b Statistical analysis of (a). Data are presented as means ± s.e.m. (n = 10 biological replicates). P values indicate the significant differences relative to ZJ1 at the indicated temperatures. Two-tailed unpaired t-test was used for statistical analysis. n.s., not significant. c Mapping and identification of wenmin1. Arrows, predicted genes; black boxes, exons; black lines, introns. Numbers indicate the exon length. The specific single nucleotide polymorphism in Tian1S, HNS-1, and ZJ1-wenmin1 was shown in red. d OsMS1-GUS is specifically detectable in the stages 8 and 9 anthers. S7, Stage 7; S8, stage 8; S9, stage 9; S10, stage 10. e Schematic diagram of OsMS1 protein structure. Green, purple and red rectangles indicate NLS, LXXLL motif, and PHD domain, respectively. aa, amino acids. The mutated amino acids (L301P) in Tian1S, HNS-1, and ZJ1-wenmin1 are shaded in red. f Spikelet (up) and pollen (down) phenotypes of ZJ1, ZJ1-wenmin1, and gOsMS1:ZJ1-wenmin1 lines (#1 and #2). Scale bars, 100 μm (a), 1 mm (d), 1 mm (f, up), 100 μm (f, down). Representative results of at least three independent experiments in (d) and (f) are shown. Source data are provided as a Source data file.