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. 2022 Apr 19;13:2055. doi: 10.1038/s41467-022-29648-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a In vitro histone pull-down assay with the indicated proteins against biotinylated peptides. Proteins were pulled down by strepavidin beads and detected by western blots with antibody against FLAG or GST. b OsMS1 associates with H4 in a LCI assay. The indicated constructs were transiently expressed in N. benthamiana at 22 °C for 48 h. Luciferase activity is depicted with false color from low (blue) to high (red). Nluc, N-terminal half of firefly luciferase; Cluc, C-terminal half of firefly luciferase. c OsMS1 associates with H4 in a BiFC assay. Assays were done as in (b). nYFP, N-terminal portion of YFP; cYFP, C-terminal portion of YFP. Black arrows indicate nuclei. Scale bars, 50 μm. d OsMS1 associates with H4 in a semi-in vivo pull-down assay. GST-OsMS1 was incubated with nuclear proteins extracts from stage 9 anthers of rice young panicles. Proteins were pulled down by Glutathione sepharose beads and detected by western blots with antibody against GST or H4. e OsMS1 associates with H4 in a co-immunoprecipitation assay. Proteins extracts from stage 9 anthers of transgenic 35S:GFP-OsMS1 rice was immunoprecipitated with a GFP-Trap^®_A agarose beads and detected by western blots with antibody against GFP or H4. 35S:GFP transgenic rice was used as a negative control. Experiments were repeated more than three times with similar results. Source data are provided as a Source data file.