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. 2022 Apr 19;13:2055. doi: 10.1038/s41467-022-29648-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Temperature-dependent association of OsMS1^wenmin1 and TDR in yeast. Yeast cells (OD₆₀₀ = 1) were spotted on the SD-4 plates and grown at 22 °C, 26 °C, or 30 °C for 96 h. b Temperature-dependent associations of OsMS1 and OsMS1^wenmin1 with TDR in LCI assays in N. benthamiana plants. N. benthamiana plants infiltrated with the Agrobacteria combinations were incubated at 22°C for 44 h and then transferred to the indicated temperatures for another 4 h. c Quantitative analysis of (b). Box-and-whisker plots display the minimum and maximum, the 25th and 75th percentiles (box), and median (center line). RLU, relative luminescence unit. Data are presented as mean RLU ± s.e.m. (n = 12 biological replicates). Two-tailed unpaired t-test was used for statistical analysis. n.s., not significant. d Temperature-dependent associations of OsMS1 and OsMS1^wenmin1 with TDR in BiFC assays in N. benthamiana plants. nYFP, N-terminal portion of YFP; cYFP, C-terminal portion of YFP. Scale bars, 1 μm. e OsMS1 and OsMS1^wenmin1 interact with TDR in a GST pull-down (PD) assay. The proteins were detected by western blots with antibody against GST or FLAG. Experiments in (e) were repeated three times with similar results. Source data are provided as a Source data file.