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. 2022 Apr 19;5:375. doi: 10.1038/s42003-022-03333-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Overlay of C-domains highlighting the conserved split β-sheet characteristic of ADPRT fold proteins. b Active site residue conservation. DT residues important for NAD⁺ binding and catalysis and corresponding residues in AT and PT are represented in stick form. NAD⁺ represented in partial transparency is modeled from PDB entry 1TOX for reference. c Dose titration of DT and DT C-domain chimeras DT(AT_C), DT(PT_C) on Vero cells (mean ± SD, n = 2); n = 3 biological replicates. d Dose titration of DT, DT(AT_C), and DT(PT_C) on HEK293T and HEK293T DPH4_KO cells (mean ± SD, n = 2); n = 3 biological replicates. Titration of protein synthesis inhibitor cycloheximide (CHX) is shown in red (inset).