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. 2022 Apr 19;5:375. doi: 10.1038/s42003-022-03333-9

Fig. 5. AT and PT are not efficiently processed.

Fig. 5

a Primary amino acid sequences of loops linking C and T domains in DT, AT, and PT. b Structures of processing sites in DT, AT, and PT in gray, green, and purple, respectively. c SDS-PAGE analysis of holotoxin processing by Vero cell lysate. d Dose titration of DT and DT activation loop chimeras DT(ATF), DT(PTF) on Vero cells (mean ± SD, n = 2); n = 3 biological replicates. e Time course of protein synthesis inhibition in Vero cells treated with 10 nM of DT, DT(ATF), and DT(PTF) (mean ± SD, n = 2); n = 3 biological replicates. f Dose titration of DT and DT (uncleavable) on Vero cells (mean ± SD, n = 4); n = 3 biological replicates.