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. 2022 Apr 19;12:6428. doi: 10.1038/s41598-022-09990-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypes and genotypes of w G0-G3 mutants. (a) Parents of the WhA line, parents of the WhA G1 and G2 crosses, and five representative WhA G3 progeny. Lateral view of eyes and ocelli, as well as forewings and hindwings, are shown for each individual. (b) The w target region, location of sgRNAw6-1 and sgRNAw6-2, sequence of wild-type and G0-G3 w mutants, and w alleles are shown. PAM site (red), sgRNAs (bold), insertions and substitutions (lower case), deletions (dashes). (c) Deduced amino acid sequences from the wild-type and w alleles spanning residues 283 to 303 of the GWSS white protein. Residues identical to the wild-type protein are highlighted in black. Termination codon (*). (d) PCR amplification products from the w target region. The 83-bp insertion of the w² allele is easily resolved. Lane N is no DNA template control.