Table 1.
Effect of 50 nM CRF on intrinsic properties of insular cortex pyramidal neurons.
| Parameter | Sex | Means (SEM) | Results | Summary | ||
|---|---|---|---|---|---|---|
| aCSF | CRF | Main effects | Interaction | |||
| Membrane potential (mV) | M | −66.65 (1.527) | −59.64 (2.007) | CRF, F1,24 = 72.93, P < 0.0001 | F1,24 = 5.124, P = 0.033 | ⇧ |
| F | −68.41 (1.189) | −64.33 (1.268) | Sex, F1,24 = 2.267, P = 0.145 | ⇧ | ||
| Rheobase (pA) | M | 1256.42 (105.92) | 1226.42 (129.94) | CRF, F1,24 = 0.282, P = 0.600 | F1,24 = 0.924, P = 0.346 | = |
| F | 1515.00 (132.76) | 1619.17 (157.49) | Sex, F1,24 = 3.573, P = 0.070 | = | ||
| Spike amplitude (mV) | M | 73.35 (3.852) | 70.99 (4.039) | CRF, F1,24 = 4.933, P = 0.036 | F1,24 = 0.263, P = 0.612 | ⇩ |
| F | 80.58 (3.764) | 76.80 (4.136) | Sex, F1,24 = 1.430, P = 0.243 | ⇩ | ||
| AP rise rate (mV/ms) | M | 181.1 (17.55) | 157.6 (14.548) | CRF, F1,24 = 18.93, P = 0.0002 | F1,24 = 0.535, P = 0 .472 | ⇩ |
| F | 229.2 (25.536) | 196.2 (26.136) | Sex, F1,24 = 2.253, P = 0.146 | ⇩ | ||
| AP half-width (ms) | M | 1.248 (0.075) | 1.357 (0.064) | CRF, F1,24 = 16.69, P = 0.0004 | F1,24 = 0.134, P = 0.717 | ⇩ |
| F | 1.125 (0.074) | 1.256 (0.098) | Sex, F1,24 = 1.121, P = 0.300 | ⇩ | ||
| After depolarization (mV) | M | 10.36 (1.365) | 8.593 (1.312) | CRF, F1,24 = 26.83, P < 0.0001 | F1,24 = 2.142, P = 0.156 | ⇩ |
| F | 12.98 (0.805) | 9.808 (0.688) | Sex, F1,24 = 1.554, P = 0.225 | ⇩ | ||
| Burst ratio | M | 0.2608 (0.052) | 0.3889 (0.055) | CRF, F1,21 = 38.82, P < 0.0001 | F1,21 = 3.769, P = 0.066 | ⇧ |
| F | 0.2153 (0.032) | 0.2825 (0.024) | Sex, F1,21 = 1.600, P = 0.220 | ⇧ | ||
| Input resistance (MΩ) | M | 130.9 (15.318) | 120.9 (14.663) | CRF, F1,24 = 5.985, P = 0.0221 | F1,24 = 2.497, P = 0.127 | ⇩ |
| F | 104.9 (13.460) | 102.8 (14.249) | Sex, F1,24 = 1.156, P = 0.293 | = | ||
| Time constant (ms) | M | 21.71 (0.946) | 20.07 (0.946) | CRF, F1,24 = 12.37, P = 0.0018 | F1,24 = 0.173, P = 0.681 | = |
| F | 24.25 (0.799) | 22.17 (0.726) | Sex, F1,24 = 4.235, P = 0.051 | ⇩ | ||
| Rectification ratio (mV) | M | 0.8343 (0.023) | 0.9014 (0.023) | CRF, F1,24 = 35.28, P < 0.0001 | F1,24 = 0.611, P = 0.442 | ⇧ |
| F | 0.8158 (0.032) | 0.9033 (0.025) | Sex, F1,24 = 0.058, P = 0.810 | ⇧ | ||
| Sag ratio | M | 1.359 (0.175) | 1.384 (0.181) | CRF, F1,24 = 1.339, P = 0.259 | F1,24 = 2.072, P = 0.163 | = |
| F | 1.555 (0.244) | 1.331 (0.231) | Sex, F1,24 = 0.065, P = 0.801 | = | ||
| sAHP (mV) | M | −655.21 (85.958) | −765.43 (84.764) | CRF, F1,24 = 3.080, P = 0.092 | F1,24 = 0.517, P = 0.479 | = |
| F | −659.41 (49.605) | −705.58 (44.492) | Sex, F1,24 = 0.092, P = 0.479 | = | ||
| Firing rate (spikes/s @250 pA) | M | 57.214 (17.354) | 51.143 (19.000) | aCRF, F1,24 = 9.611, P = 0.005 | aF1,24 = 0.440, P = 0.503 | ⇩ |
| F | 52.092 (16.433) | 38.050 (14.649) | aSex, F1,24 = 0.010, P = 0.921 | = | ||
Data were analyzed with a two-way analysis of variance. Summary indicates the effect of CRF and sex on parameter measured. Arrows indicate significant differences between aCSF and CRF conditions (Ps < 0.05, Sidak-corrected t-test).
aThe distribution of firing rates were negatively skewed so the data were converted with a log transform prior to analysis with ANOVA.