Skip to main content
. 2022 Apr 19;13(4):372. doi: 10.1038/s41419-022-04841-6

Fig. 2. DHEA promotes Nig-induced pyroptosis in LPS-primed macrophages.

Fig. 2

A Cells were pre-treated with different doses of DHEA (0, 10, 20, 50 μM) for 1 h and primed with LPS for 4 h; then stimulated with nigericin (Nig) for 1 h. Cell morphology was observed by light microscopy, the red arrows represent the cells that burst due to pyroptosis, scale bar = 100 μm. B The GSDMD-NT protein levels were analyzed by immunofluorescence, scale bar = 50 μm. C, D PI-positive dead cells in 5 randomly selected fluorescence microscope images were counted by Image J software, scale bar = 200 μm. E The cell death was measured by detecting the lactate dehydrogenase (LDH) release (%) in cell culture supernatant. F IL-1β content in cell culture supernatant. Data are presented as means ± SEM (n = 4). *P < 0.05, **P < 0.01, compared with the respective control.