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. 2022 Apr 19;13(4):372. doi: 10.1038/s41419-022-04841-6

Fig. 3. DHEA induces abnormal autophagy and excessive release of ROS in Nig-treated inflammatory macrophages.

Fig. 3

A After indicated treatments, the p62 and LC3 protein levels were analyzed by western blotting and quantified by Image J software. B, C The formation of p62/LC3 puncta was analyzed by immunofluorescence, scale bar = 50 μm. D The intracellular ROS levels were detected using DCFH-DA, scale bar = 200 μm. Data are presented as means ± SEM (n = 3). *P < 0.05, **P < 0.01, compared with the respective control.