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. 2022 Apr 19;13(4):372. doi: 10.1038/s41419-022-04841-6

Fig. 4. DHEA activates GPER and AMPK/mTOR signal in inflammatory macrophages.

Fig. 4

A J774A.1 cells were pre-treated with DHEA (50 μM) in the presence or absence of the GPER inhibitor G15 (1 μM) for 1 h, then stimulated with LPS for 4 h. The TLR4, p-ERK/ERK, LC3, p62, p-mTOR, and p-AMPK protein expression levels were measured by western blotting and quantified by Image J software. B, C J774A.1 and RAW264.7 cells were pre-treated with G1 (1 μM) for 1 h, then stimulated with LPS for 4 h, and the indicated protein expression levels were measured by western blotting and quantified by Image J software. Data are presented as means ± SEM (n = 3 or 4). *P < 0.05, **P < 0.01, compared with the respective control.