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. 2022 Apr 19;13(4):372. doi: 10.1038/s41419-022-04841-6

Fig. 6. DHEA exacerbates Nig-induced pyroptosis in LPS-primed macrophages via GPER activation.

Fig. 6

A J774A.1 cells were pre-treated with DHEA (50 μM) in the presence or absence of the GPER inhibitor G15 (1 μM) for 1 h, then stimulated with LPS for 4 h, PI-positive dead cells in 5 randomly selected fluorescence microscope images were counted by Image J software, scale bar = 200 μm. B The GSDMD-NT protein levels were analyzed by immunofluorescence, scale bar = 50 μm. C The p62 puncta formation was analyzed by immunofluorescence, scale bar = 50 μm. D The cleaved caspase-8 protein levels were analyzed by immunofluorescence, scale bar = 50 μm. E Possible mechanism by which DHEA exacerbates cell death. Data are presented as means ± SEM (n = 3). **P < 0.01, compared with the respective control.