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. 2022 Apr 19;12:6455. doi: 10.1038/s41598-022-10428-0

Figure 3.

Figure 3

The expression patterns of V2Rs in the olfactory epithelium of the basal ray-finned fish, Polypterus senegalus. Overall view of a HE-stained horizontal section of the olfactory organ, showing that lamellae of olfactory organ are stained blue-violet, and the nerve bundles are stained red-violet (A). Expression of four V2R genes were confirmed by FISH on horizontal sections of the olfactory organ of the bichir using DIG-labeled RNA antisense probes for f-V2R (B) and t-V2R (C), V2R2 (D), and ancV2R (E). Green indicated the expression signals. The blue area indicates the cell nucleus stained with DAPI. V2R2 was globally expressed in the deep layers of olfactory lamellae (D). In contrast, f-V2R, t-V2R, and ancV2R were sparsely expressed in a small number of neurons in the deeper layers of the olfactory lamellae (B, C, and E). (B’-E') High magnification view of the dotted squares in B–E. Scale bars show 1 mm (A), 100 µm (B–E) and 20 µm (B'–E').