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. 2022 Apr 19;12:6469. doi: 10.1038/s41598-022-09957-5

Table 1.

PCR primer sequences used for RT–PCR.

mRNA Forward primer (5′-3′) Reverse primer (5′-3′) Accession number Size Tm
CRALBP CCTCAACTGTCCTGGACCCAAGGC TTACCCATCCCCCAACTTGAGA NM_000326 123 58
RPE-65 GCCCAGGAGCAGGACAAAAG GCGCATCTGCAAGTTAAAACCA NM_000329 247 52
GAPDH CCCCACCACACTGAATCTCC GGTACTTTATTGATGGTACATGACAAG NM_002046 104 60