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. 2022 Apr 19;13:2056. doi: 10.1038/s41467-022-29741-3

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© The Author(s) 2022, corrected publication 2024

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Fig. 2 — A Serial sections of multiciliated cells were performed from 7 different cells (one embryo) fixed at stage 20-21 and from 5 different cells (one embryo) fixed at stage 30. Ten cilia were analyzed at each stage by transmission electron microscopy (scale bars, 500 nm). B 3D reconstructions were performed, using Reconstruct (Movie 1). C The minimal angle between rootlets and basal bodies (upper panel), and the minimal distance from the tip of the basal body rootlet to the plasma membrane (lower panel) significantly decreased between stage 20-21 and stage 30 (see Movie 2 for the applied workflow) (mean ± SD; Mann–Whitney-U test). Source data are provided as a Source Data file.